3Dpol-F1 |
TTTCATCGTCGGCACTCACTC |
Sequencing of the full-length 3Dpol-encoding gene |
3Dpol-F2 |
TGCGCTGATTGACTTCGAGAAC |
|
3Dpol-R |
GGAATGTGGAAGCGGGAAAAG |
|
D5N-mu-F |
CGAGGGATTGGTTGTTAACACCAGAGATGTGGG |
Production of the D5N mutant |
D5N-mu-R |
CTCCACATCTCTGGTGTTAACAACCAATCCCTCG |
|
A38V-mu-F |
GAATTTGGGCCTGCTGTCTTGTCCAACAAGGAC |
Production of the A38V mutant |
A38V-mu-R |
GTCCTTGTTGGACAAGACAGCAGGCCCAAATTC |
|
M194I-mu-F |
CACATTCTTTACACCAGGATTATGATTGGCAGATTTTGGC |
Production of the M194I mutant |
M194I-mu-R |
GCACAAAATCTGCCAATCATAATCCTGGTGTAAAGAATGTG |
|
M296V-mu-F |
CATTGTTGTTGAGGGCGGGGTGCCGTCTGGCTGTTCCGC |
Production of the M296V mutant |
M296V-mu-R |
GCGGAACAGCCAGACGGCACCCCGCCCTCAACAACAATG |
|
Real-time-F |
AATGCACTCAAACAACGGAC |
Quantitative real-time PCR for detecting RNA synthesis |
Real-time-R |
GCAGTGGTTAGCATCAAAGG |
|
Di-Fitness-F1 |
TTTCATCGTCGGCACTCACTC |
Amplification of 3D for the direct fitness assay |
Di-Fitness-F2 |
TGCGCTGATTGACTTCGAGAAC |
|
Indi-Fitness-F |
CGCGCAAGAGACAGCAGATGG |
Amplification of 3A to 3D for the indirect fitness assay |
Indi-Fitness-R |
GGAATGTGGAAGCGGGAAAAG |
|
Mu-frequency-F |
TGTCCAACCTTCCTCCGCTTC |
Amplification of VP3 for the mutation frequency assay |