FIG 1.

Gly-9 effects on PrPres formation. (A) Immunoblotting of PrPres in three distinct prion strain-infected cell lines (ScN2a, N167, and F3) treated with Gly-9. Cells were treated for 3 days with the indicated dose of Gly-9. β-Actin signals are shown as controls for the integrity of samples used for PrPres detection. Graphic data are averages and standard deviations for triplicate immunoblot signals. (B) Immunofluorescence of abnormal PrP accumulation in Gly-9-treated N167 cells. Cells were treated with 5 μg/ml Gly-9 or its vehicle (DMSO) for 3 days. Nuclei were stained with Hoechst 33258 (H33258). (C) Temporal profile of PrPres levels in Gly-9-treated N167 cells. Cells were treated with 10 μg/ml Gly-9 or its vehicle for the indicated periods. Graphic data are averages and standard deviations for triplicate immunoblot signals (n.s., not significant; *, P < 0.01).