FIG 4.

Differential sensing of SIV replication by PBMCs. (A) Example for one experiment. Human PBMCs were infected by SIVwt or SIVopt1. IFN-I induction was measured with the profile of intracellular MxA expression, an interferon-stimulated gene. SIVopt1 shows a reduced ability to induce MxA expression compared to SIVwt. Rebound at day 14 is likely caused by cell mortality. (B) Box plot of SIVwt and SIVopt1 for every measured MxA expression point from 7 different human PBMC donors infected by SIVopt1 and SIVwt. Each PBMC donor sample was divided into several cultures that were infected with different viral input concentrations (always paired between SIVwt and SIVopt1). MxA expression was measured at regular time points following infection (see Fig. S3 in the supplemental material). SIVopt1 globally stimulated less MxA synthesis than SIVwt; ***, paired Wilcoxon test, P = 3. 10⁻⁵ on more than 150 pairs.