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Enhancement of the infectivity of gRNA by S1b or S2b mutant N mRNA. L2 cells were cotransfected by electroporation with wild-type MHV gRNA plus synthetic N mRNA or a water control (18). Transfected cells were seeded into dishes and overlaid with agar, and plaques were counted after 48 h of incubation at 37°C. Each histogram represents the mean infectious titer (±standard deviation) from four separate transfections.
