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. 2014 Apr;88(8):4275–4290. doi: 10.1128/JVI.03287-13

FIG 5.

FIG 5

Ectopic expression of PtdSer receptors on 293T cells. (A) Schematic representation of lentiviral vectors expressing TIM-1, -3, and -4, CD300a, or BAI1 and plasmid vectors expressing stabilin-1 and -2. SIN LTR, self-inactivating long terminal repeat; Ubc pro, ubiquitin C promoter; SV40 pro, SV40 promoter; TK, thymidine kinase. (B) Expression of Axl, TIM-1, -3, and -4, CD300a, BAI1, and stabilin-1 and -2 on 293T cells ectopically expressing each of these receptors analyzed by the use of antibodies specific to each receptor. Black line, staining by control antibodies; red line, staining by antibodies specific to each PtdSer receptors. (C) (Left column and top four panels of right column) Expression of TIM-1, -3, and -4, CD300a, BAI1, and stabilin-1 and -2 on 293T cells ectopically expressing each of these receptors and parental 293T cells analyzed by the antibody specific to the Flag tag. Black line, staining by APC-conjugated isotype control antibody; red line, staining by APC-conjugated anti-Flag tag antibody. (Bottom two panels of right column) Expression of Axl was analyzed by incubating 293T and Axl 293T cells with hGas6, followed by staining with the anti-Flag tag antibody. Black line, staining by APC-conjugated anti-Flag tag antibody without preincubation with hGas6; red line, staining by APC-conjugated anti-Flag tag antibody after incubation with hGas6.