FIG 7.

Quantitation of splenic colonization by eGFP⁺ MuHV-4. (a) We infected C57BL/6J (WT) or BAFF-R^−/− mice i.p. with eGFP⁺ MuHV-4 as for Fig. 5 and 6 and after 4 or 8 days typed eGFP⁺ cells as macrophages (CD169⁺ or MARCO⁺) or B cells (IgM⁺ or IgD⁺). Each bar shows the mean ± SD for 3 sections from each of 3 mice. Macrophage infection was not significantly different between the mouse groups, but WT mice showed significantly more B cell infection at day 4 (P < 0.001). (b) EGFP⁺ B cells at day 8 were further subdivided into IgM^hi IgD^lo (predominantly marginal-zone) and IgM^lo IgD^hi (predominantly follicular) populations. The eGFP⁺ B cells of WT mice were significantly higher in IgM^lo IgD^hi and significantly lower in IgM^hi IgD^lo than those of BAFF-R^−/− mice (P < 0.001).