FIG 8.

Colonization of GC B cells by eGFP⁺ MuHV-4. (a) We infected C57BL/6J (WT) or BAFF-R^−/− mice i.p. with eGFP⁺ MuHV-4 (10⁵ PFU) and 8 days later stained splenic sections for eGFP (green) and PNA (red). Nuclei were stained with DAPI (blue). Arrows show eGFP⁺ PNA⁺ cells. The boxed areas in the left-hand overview images (scale bar = 100 μm) are shown at higher magnification, with separate and merged channels in the right-hand images. No eGFP⁺ PNA⁺ cells were seen in BAFF-R^−/− spleens. (b) Mice were infected as for panel a, and eGFP⁺ cells were scored as inside or outside PNA⁺ follicles. Each bar shows the mean ± SD for 3 sections from each of 3 mice. In WT mice, a significantly higher proportion of the eGFP⁺ cells were inside follicles than in BAFF-R^−/− mice (P < 0.0001).