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. 2014 Apr;88(8):4558–4571. doi: 10.1128/JVI.03431-13

FIG 1.

FIG 1

Effects of Neu5Acα2Me and antibody to α2β1 integrin on human rotavirus infection of untreated and sialidase-treated MA104 cells. (A) Chemical structures of monomeric N-acetylneuraminic acid and its corresponding methyl glycoside. (B to E) Rotavirus reacted with 10 mM Neu5Acα2Me or diluent (control) was adsorbed to cells treated with sialidase (0.52 U/ml) and/or α2β1 antibody (20 μg/ml). Infected cells were enumerated at 16 h postinfection following indirect immunofluorescent staining. Infectious virus titers are expressed as a percentage of the titers produced in control untreated cells. These control infectious titers, expressed as mean FCFU/ml ± SD, were 7.7 × 103 ± 1.3 × 103 (B), 1.2 × 104 ± 0.1 × 104 (C), 8.8 × 103 ± 0.7 × 103 (D), and 1.6 × 104 ± 0.3 × 104 (E). Positive-control neutralizing monoclonal antibodies (Ab) were 1A10 (Wa), RV-5:2 (RV-5), and RV-3:1 (RV-3; S12/85). Cells treated with isotype control MOPC21 at 20 μg/ml produced virus titers that were indistinguishable from those of untreated cells.