FIG 2.

TRIM5α and TRIM22 expression in different immune cells. (A) Representative flow cytometry plots showing the gating strategy employed to define different cell populations. (Top) Cells were first gated for singlets (forward scatter height [FSC-H] versus forward scatter area [FSC-A]), and CD4⁺ T cells were defined from the lymphocyte gate (side scatter area [SSC-A] versus FSC-A) as CD3⁺ CD4⁺ events. (Middle) Flow cytometry plots illustrate the gating for B cells as CD3⁻ CD19⁺ cells and monocytes as CD3⁻ CD19⁻ CD56⁻ CD14⁺ cells. (Bottom) NK cells were defined as CD3⁻ CD56⁺ CD16⁺ cells. (B) Data were compared between each subset of cells in the CSF and PBMCs only by using the paired t test. Magnetic cell sorting was employed to isolate CD4 cells and monocytes from 6 fresh HIV-1-negative PBMC samples. Natural killer cells were isolated by using the Easy Sep negative-selection human NK cell enrichment kit (Stemcell Technologies). (C and D) Different cell populations were analyzed for TRIM5α (C) and TRIM22 (D) mRNA expression. Data are depicted as normalized ratios of TRIM5α or TRIM22 to GAPDH.