FIG 4.

Regulation of TRIM5α and TRIM22 by IFN-α and select proinflammatory cytokines in immune cells. Shown is the effect of IFN-α stimulation and proinflammatory cytokine stimulation of CD4 cells and monocytes on antiviral gene expression. Cells from healthy donors were stimulated for 24 h. (A and B) Total cellular RNA from cells was then subjected to real-time RT-PCR to measure mRNA levels using primers specific for TRIM5α (A) and TRIM22 (B) and GAPDH. (C to E) Protein expression analyses of TRIM5α (C and D) and TRIM22 (C and E) are shown. Results shown in panels A, B, D, and E are the means of three independent experiments (with bars indicating ranges), each performed in duplicate.