FIG 9.

Virus-induced vesicles contain pores open to the cytoplasm. Electron tomography of DENV-infected C6/36 cells shows Vp pores. (A) The images suggest that the neck of the Ve gives rise to the Vp pores. (B) Membrane connection of Ve pores and budding vesicles. (C and E) The 3D surface-rendered model of DENV-infected C6/36 cells. (C) Structures of Ve membrane (top orange) and budding vesicles (bottom orange) demonstrate the position of a pore and the connection between the two membranes vesicles (derived from panel B). (D) Close association of two different Vp membranes containing the Ve and virus particles. (E) The 3D model shows close association between two independent Vp membranes (yellow) that contain Ve (orange) and a virus particle (red) (derived from panel D). The RC structure (blue) is oriented in the direction of the Ve pore. See Movie S3 in the supplemental material for the reconstruction tomogram and 3D rendering.