FIG 3.

Origin of BFV miRNAs. (A) Predicted sequence and RNA secondary structure of the BFV pri-miRNA. Black triangles indicate the sites of cleavage during BFV pri-miRNA processing to yield the two viral pre-miRNAs. (B) Paired-end deep sequencing of BFV sequences of ≥30 nt. The y axis shows the number of reads containing a given nucleotide divided by the total number of viral reads in the library. Values obtained with the forward sequencing primer are plotted on the positive y axis, with values obtained with the reverse primer plotted on the negative y axis. (C) Proviral DNA sequence with potential Pol III transcription factor binding sites and termination sequences boxed. Numbers represent the nucleotide locations within the reference BFV strain.