TABLE 1.
Summary of BFV small RNAs recovered from chronically BFV-infected MDBK cellsa
miRNA | Sequence | Length (nt) | Start position | End position | No. of reads | % of reads |
---|---|---|---|---|---|---|
miR-BF1-5p | UUCGGAGGAUGGCUCAUCAAGC | 22 | 11,190 | 11,211 | 878,086 | 32 |
UUCGGAGGAUGGCUCAUCAAGCU | 23 | 11,190 | 11,212 | 836,116 | 30 | |
UUCGGAGGAUGGCUCAUCAAG | 21 | 11,190 | 11,210 | 659,169 | 24 | |
UUCGGAGGAUGGCUCAUCAAGCC | 23 | 11,190 | 11,212 | 127,154 | 5 | |
miR-BF1-3p | UCCCUGAAGCCAUAUCCGAGGC | 22 | 11,224 | 11,245 | 86,127 | 58 |
UCCCUGAAGCCAUAUCCGAGG | 21 | 11,224 | 11,244 | 28,224 | 19 | |
UCCCUGAAGCCAUAUCCGAGGU | 22 | 11,224 | 11,245 | 6,142 | 4 | |
UCCCUGAAGCCAUAUCCGAGGCU | 23 | 11,224 | 11,246 | 3,400 | 2 | |
UCCCUGAAGCCAUAUCCGAGGCA | 23 | 11,224 | 11,246 | 3,185 | 2 | |
miR-BF2-5p | UCAGUAGAAAGACAGUACCUCGCC | 24 | 11,246 | 11,269 | 3,514,215 | 47 |
UCAGUAGAAAGACAGUACCUCGCCU | 25 | 11,246 | 11,270 | 2,052,969 | 28 | |
UCAGUAGAAAGACAGUACCUCGCCC | 25 | 11,246 | 11,270 | 384,581 | 5 | |
UCAGUAGAAAGACAGUACCUCGC | 23 | 11,246 | 11,268 | 369,154 | 5 | |
UCAGUAGAAAGACAGUACCUCGCCUGU | 27 | 11,246 | 11,272 | 301,059 | 4 | |
miR-BF2-3p | CCAGGCGGUAUGCUUUCUACU | 21 | 11,285 | 11,305 | 35 | 58 |
CCAGGCGGUAUGCUUUCUACUU | 22 | 11,285 | 11,306 | 11 | 18 | |
UCAGGCGGUAUGCUUUCUACU | 21 | 11,285 | 11,305 | 3 | 5 | |
CCAGGCGGUAUGCUUUCUAC | 20 | 11,285 | 11,304 | 2 | 3 | |
CCAGGCGGUAUGCUUUCUACUUUU | 24 | 11,285 | 11,308 | 2 | 3 |
RNA species that contributed ≥2% of the total reads for a given BFV miRNA are shown, with their positions relative to the reference BFV strain (accession no. JX307862.1) indicated by genome coordinates. Nontemplated nucleotides are indicated in bold.