TABLE 2.
BFV miRNAs are expressed in vivoa
| Sample | BFV infection | ΔΔCT value |
|
|---|---|---|---|
| BF1-5p | BF2-5p | ||
| Calf A PBLs | + | 1.00 | 1.00 |
| Calf B PBLs | + | 1.21 | 1.05 |
| Calf C PBLs | + | 2.02 | 1.97 |
| Calf D PBLs | − | 0.014 | ND |
| Calf E PBLs | − | 0.003 | ND |
| MDBK cells | − | 0.001 | ND |
| Acute | 2.68 | 10.7 | |
| Chronic | 3,880.00 | 10,390.00 | |
a
Relative expression of miR-BF1-5p and miR-BF2-5p in PBLs from three BFV-infected calves (+) (A, B, and C) and two uninfected calves (−) (D and E), as well as in uninfected, freshly infected, and chronically infected MDBK cells. Expression was calculated by the ΔΔCT quantitative PCR method (26), using the endogenous U6 snRNA as the reference, with the value for PBLs from calf A arbitrarily set to 1.0. ND, no detectable amplification.