TABLE 1.

Lysis time of N-terminal mutants of the lysis protein E

Genomea	Protein E	Lysis timec (min)
WT	WT	24.5 ± 0.2
T572C	V2A	36.2 ± 0.6***
T572A	V2E	27.8 ± 0.2*
T572G	V2G	NDb
G575A	R3H	24.4 ± 1.0
G575C	R3P	38.0 ± 0.2**
G575T	R3L	23.6 ± 0.3
G578C	W4S	ND
G578T	W4L	27.6 ± 0.4*
C581T	T5I	23.2 ± 0.1*
T584C	L6S	23.0 ± 0.6*
G587T	W7L	35.9 ± 1.1**
G587C	W7S	36.3 ± 0.8***
A590T	D8V	ND
A590C	D8A	38.5 ± 1.0***
A590G	D8G	ND
C593T	T9I	33.8 ± 1.0**

^aNumbering is according to that of GenBank accession number GQ153915.

^bND, lethal or highly deleterious mutant, based on titration.

^cAsterisks indicate values significantly different from those for the WT (*, P < 0.05; **, P < 0.01; ***, P < 0.001, as determined by the t test).