TABLE 3.
Mutation rate estimates from fluctuation tests in the presence/absence of 10 ng/μl 5-FU
| Virus and treatment | No. of testsa | N0b (PFU) | N1c (PFU, ×10−5) | P0d | μe (s/n/r, ×106) |
|---|---|---|---|---|---|
| None | |||||
| WT | 4 | 110, 432, 178, 323f | 5.2, 3.6, 2.3, 13.1 | 0.33, 0.46, 0.58, 0.17 | 0.864 ± 0.096 |
| V2Ag | 3 | 192, 382, 468 | 3.6, 1.8, 1.4 | 0.38, 0.67, 0.58 | 1.26 ± 0.19 |
| D8Ag | 5 | 743, 397, 198, 305, 397 | 9.1, 5.0, 6.2, 3.2, 6.6 | 0.17, 0.67, 0.58, 0.79, 0.63 | 0.436 ± 0.102 |
| 5-FU | |||||
| WT | 6 | 213, 90, 157, 375, 343, 462 | 0.042, 0.092, 0.087, 0.15,0.23, 0.084 | 0.83, 0.67, 0.71, 0.17, 0.25, 0.54 | 27.9 ± 5.4 |
| V2Ag | 3 | 235, 140, 252 | 0.13, 0.15, 0.13 | 0.75, 0.75, 0.79 | 8.73 ± 0.59** |
| D8Ag | 3 | 687, 407, 368 | 0.025, 0.099, 0.092 | 0.96, 0.58, 0.75 | 16.2 ± 4.2 |
a
Number of independent fluctuation tests performed.
b
Initial number of PFU per culture.
c
Final number of PFU per culture.
d
Fraction of 24 cultures showing no gro87-resistant plaques.
e
For each test, the mutation rate was calculated as μ = [−lnP0/(N1 − N0)] × (3/Ts), where Ts = 7.
f
Fluctuation tests were performed in two experimental blocks (differentiated by underlining).
g
Asterisks indicate significant differences in mutation rates in a t test against the WT (**, P < 0.01).