FIG 6.

Blockade of dendritic cell-lymphocyte cross talk by anti-ICAM-1. Anti-ICAM-1 antibody (1H4; 20 μg/ml) was added to HIV-1-infected or uninfected MoDCs prior to coculture with autologous lymphocytes. At 48 h postinfection, intracellular HIV-1 infection (A) and SAMHD1 expression (B) were measured and expressed as a percentage of the p24⁺ or SAMHD1⁺ DC-SIGN⁺ MoDCs compared to control cells in the absence of coculture. The percent decrease of HIV-1 infection was calculated and is shown in red. The data are means ± SD from two independent healthy blood donors.