FIG 4.

Ikaros regulates the levels of some key players in B-cell differentiation. (A and B) Changes in levels of the indicated cellular transcription factors following knockdown (A) or overexpression (B) of Ikaros. (A) EBV⁺ MutuI cells were infected for 3 days with lentivirus expressing nontargeting shRNA (Control #1) or a combination of five shRNAs targeting Ikaros (Ikaros) and then incubated for 5 days in the presence of puromycin. Whole-cell extracts were processed for immunoblot analyses. (B) MutuI cells were infected for 4 days with lentivirus 525 expressing IK-1 (IK-1) or with the empty vector (Control) prior to harvesting for immunoblot analyses. (C) Differences in mRNA levels of some key transcription factors in memory B and plasma cells. Expression levels in memory B cells and in vitro-generated plasma cells and bone marrow plasma cells were visualized with Expression Atlas (experiment E-MEXP-2360; http://www-test.ebi.ac.uk/gxa/experiment/E-MEXP-2360/ENSG00000185811/cell_type) (74). Arrows indicate significant up- and downregulation. Error bars indicate maximum and minimum values; top of light, medium, and dark regions of each bar indicates 75th, 50th, and 25th percentile, respectively.