FIG 1.
NaF uptake and inflammatory cell infiltration in the CNSs of mice infected with laboratory-attenuated CVS-B2c or wt DRV-Mexico. Female ICR mice were infected i.c. either with 1 or 10 LD50 of CVS-B2c or with 10 or 100 LD50 of DRV-Mexico. (A) At day 6 or 9 p.i., BBB permeability was assessed by NaF uptake in the cerebrum and the cerebellum. (B) CD3+ T cells were quantified and analyzed statistically. Data are means ± SEM of results from three independent experiments. Statistical analyses for panels A and B were performed using one-way ANOVA followed by Tukey's post hoc test. Asterisks indicate statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). (C) Harvested brains were subjected to HE staining for histopathology as well as to IHC for the detection of CD3+ T cells.