FIG 3.

Infection of human or mouse BMECs with RABVs and their effects on TJ protein expression. (A) Human or mouse BMECs were cocultured with CVS-B2c or DRV-Mexico at an MOI of 1, and cells were fixed at 120 h after inoculation to assess active viral replication by using FITC-conjugated anti-RABV N antibodies. mNA cells infected with each virus were included as positive controls. Phase-contrast microscopy was performed to ensure cell viability and structure. (B) Viral RNA from mouse or human BMECs infected with each virus was detected at 0, 24, 48, and 120 h by quantitative RT-PCR. (C) The expression of TJ proteins on BMECs cocultured with either virus was detected by using confocal microscopy and antibodies to TJ proteins. BMECs cocultured with the supernatant of mNA cells infected with rHEP-CXCL10 were included as a positive control.