FIG 2.

SIVmac251 and SIVmac239 with N173 replicate well in rhesus macaque PBMC but poorly in rhesus macaque macrophages. (A) All SIVmac251 and SIVmac239 recombinant viruses replicated at high levels in rhesus PBMC, while SIVmac316 replication in these cells was reduced by the N173Q mutation. (B) Addition of N173 reduced SIVmac251 replication in macrophages, while mutation of N173 enhanced SIVmac239 replication in macrophages. SIVmac316 N173Q replicated efficiently in rhesus macrophages but at slightly lower levels than those of SIVmac316. (C) Infection of SIVmac251 and SIVmac239 N173Q induced MNGC (arrows) in MDM. Viruses used for infection were normalized by the p27 concentration (10 ng p27). Shown are means and standard deviations of samples from duplicate wells.