FIG 5.

Mutation of N173 impairs single-round SIV infection of Affinofile cells. (A) Viral proteins in SIV virions were analyzed by Western blotting. (B) Affinofile cells were induced with doxycycline and ponasterone to express different levels of human CD4 and CCR5. Levels of expression were analyzed by flow cytometry and quantified by using QuantiBRITE. ABS, antibodies. (C) SIVs with Envs lacking N173 showed impaired infectivity compared to those with Envs with N173 and did not mediate CD4-independent infection in Affinofile cells.