FIG 1.
(A) FMDV 3Cpro reduces the transcript levels of ISGs. HeLa cells were transfected with pXJ41, pXJ41-GST, pXJ41-NS1, pXJ41-3ABC, pXJ41-3A, pXJ41-3B, or pXJ41-3C and treated with 1,000 U/ml of IFN-β at 24 h posttransfection. Five hours later, the transcript levels of ISGs were analyzed by real-time RT-PCR. pXJ41-GST and pXJ41-NS1 were used as negative and positive controls, respectively. Relative transcript levels are shown as relative fold changes in comparison with the level for the mock-treated control of untransfected cells. The data represent the means of three independent experiments, with each experiment performed in triplicate. Data represent the means ± the standard deviations (error bars) of three experiments. Expression of 3ABC, 3A, 3B, or 3C protein in HeLa cells transfected with pXJ41-3ABC, pXJ41-3A, pXJ41-3B, or pXJ41-3C was detected by Western blotting using FLAG antibody (B) or HA antibody (C).