FIG 9.
The protease activity of FMDV 3Cpro is responsible for KPNA1 degradation. (A) HeLa cells were transfected with empty vector pXJ41, pXJ41-3C, or individual mutants of pXJ41-3C. After 24 h, the endogenous KPNA1 was tested by Western blotting using antibody against KPNA1, HA, or β-actin. The data presented here are results from one experiment of three Western blotting experiments. (B) Densitometry analysis of the digital image of KPNA1 from three independent experiments. The band intensities of blotting with KPNA1 antibody are shown as the relative protein expression levels, normalized with β-actin. Data represent the means ± the standard deviations (error bars) of three experiments.