FIG 3.

Effect of viral mutation S173T on epitope recognition of HIV-infected cells by KF11-specific CD8⁺ T cells. Ex vivo CD4⁺ T cells from B*57:03⁺ and B*57:03⁻ donors were infected with wild-type NL43 virus or NL43 virus harboring the S173T viral mutation. Infected CD4⁺ T cells were then cultured with KF11-specific CD8⁺ T cells (A) or IAW9-specific CD8⁺ T cells (B), and the level of CD8⁺ T-cell activation was monitored by expression of CD107 and Mip1β. Data from both experiments were standardized relative to percent recognition by wild-type virus (C). Experiments were performed in triplicate; means and standard deviations are shown. Student's t test was performed. *, P < 0.01; **, P < 0.001; ***, P < 0.0001. NS, not significant.