FIG 5.

Viral replication capacity of NL43_GFP virus with multiple B*57:03-associated viral mutations. NL43_GFP virus was engineered to contain combinations of the viral mutations Thr-173, Gly-163, and Asn-165. MT4 cells were infected and monitored for GFP-positive cells over 14 days (A). The slope of the curve was calculated from the exponential growth phase using the LOGEST function and converted to natural logs (B). Experiments were performed in triplicate; mean and standard deviations are shown. Dunnett's multiple-comparison tests were performed. *, P < 0.01; **, P < 0.001; ***, P < 0.0001.