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. 2014 May;88(9):4632–4646. doi: 10.1128/JVI.00014-14

FIG 3.

FIG 3

Replication efficiency of wt and individual mutant viruses in MDCK and MDCK-V2 cells. (A) The efficiency of replication of wt and various mutant viruses was evaluated by determination of maximal titers after low-multiplicity infections in either MDCK-V2 and MDCK cells, as indicated in Fig. 2. Values are averages and standard deviations from 3 experiments. *, P < 0.05; **, P < 0.01. (B) Kinetics of virus multiplication at low MOI. The titers at various times postinfection of MDCK-V2 (black lines) or MDCK (gray lines) cells were determined by plaque assay in MDCK-V2 cells. (C) Plaque size analysis of wt or mutant viruses. Wt or mutant viruses (mutant 2 or ΔNS1) were used for plaque assay in parallel in MDCK-V2 or MDCK cell cultures. After staining, the plaque size was determined using Image J software. The numbers of plaques measured were 176 (wt), 229 (ΔNS1), and 116 (mutant 2) in each cell line. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.