FIG 5 .

The absence of a suitable Φ187 receptor prevents Φ187-mediated import of SaPIs to classical S. aureus. Phage Φ187-mediated transfer of SaPIbov1 or SaPI187β to the S. aureus PS187 strain panel, including GN1 lacking WTA GalNAc modification and S. aureus RN4220 wild type and RN4220-H. SaPI donor strains were VW1 (SaPIbov1) and VW7 (SaPI187β). Values represent the ratio of transduction units (TRU; transductants/ml phage lysate) to PFU (plaques/ml phage lysate on S. aureus PS187 w.t.) and are given as means (n = 3 experiments) ± SD. No TRU were observed in controls lacking phages or SaPI particles. PS187 and RN4220 wild type (w.t.), tagN mutant (GN1), tagN-complemented GN1 mutant (c-GN1 [tagN]) and RN4220-H expressing ST395 WTA are indicated.