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. 2014 Apr 15;5(2):e00990-14. doi: 10.1128/mBio.00990-14

FIG 1 .

FIG 1 

Dose, CFU, and time dependence of CODH activity using 13CO. (A) M. bovis BCG (3 ml, 1 × 107 CFU/ml) was incubated with increasing doses of 13CO at 37°C for 4 h. Headspace δ-13CO2 was determined by IRMS. Numbers are means ± SD (n = 4) of results from separate biological replicates. By one-way ANOVA with the Tukey test as a post hoc test, P was <0.001 (0 ml versus 2 or 4 ml). (B) Different dilutions of M. bovis BCG (3 ml) were incubated with 2 ml 13CO for 4 h. Headspace δ-13CO2 was determined by IRMS. Numbers are means ± SD of results from separate biological replicates with 1 × 105, 1 × 107, or 1 × 108 CFU (n = 3) or 0 or e6 CFU (n = 2). By one-way ANOVA with the Tukey test as a post hoc test, P was <0.05 (1 × 105 versus 1 × 107 and 1 × 108; the significance for 1 × 106 cannot be determined). (C) M. bovis BCG (3 ml, 1 × 107 CFU/ml) was incubated with 2 ml 13CO for differing time points. Headspace δ-13CO2 was determined by IRMS. Numbers are means ± SD (n = 4) of results from separate biological replicates. By one-way ANOVA with the Tukey test as a post hoc test, P was <0.05 (0 h versus 0.5, 1, 3, or 5 h).