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. 2014 Apr 22;5(2):e01017-13. doi: 10.1128/mBio.01017-13

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Copyright © 2014 Wichmann et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — Organization of genes on ten metagenomic fosmid clones conferring resistance to kanamycin (KM), tetracycline (TC), carbenicillin (CAR), or ceftazidime (CTZ) assessed using Pacific Biosciences RS sequencing technology. Genes are as follows: nat, N-acetyltransferases; aph, aminoglycoside phosphotransferases; aacA-aphD, bifunctional aminoglycoside-modifying enzyme; tetW and tetO, tetracycline resistance genes; cat, chloramphenicol acetyltransferase; bla, beta-lactamases. Genes with annotations related to mobile genetic elements are labeled as IS (insertion sequence elements/transposases) or parB (plasmid-partitioning protein). Identical genes are connected with gray lines. Antibiotic resistance genes for which we demonstrated a function by subcloning are indicated with gray boxes and asterisks. All other labels were assigned based on sequence homology.