Figure 4.

Monitoring the cellular uptake and metabolism of peracetylated ManNAc derivatives in Jurkat cells. (A) Monitoring the cellular uptake and metabolism of Ac₄ManNAl in Jurkat cells by treating cells with 50 μM Ac₄ManNAl for 1, 5, or 10 min, followed by growing the cells in Ac₄ManNAl-free medium for 5 or 21 h. (B) Monitoring the cellular uptake and metabolism of peracetylated ManNAc derivatives in Jurkat cells by a pulse-chase labeling. Pulse, Ac₄ManNAl (50 μM, 1 min); Chase, Ac₄ManNAl-free medium with or without the addition of Ac₄ManNAz. The red curve represents the alkyne-dependent MFI at various time intervals when chasing with an unnatural sugar-free medium. The blue curve represents the alkyne-dependent MFI at various time intervals when chasing with 50 μM Ac₄ManNAz. (C) Monitoring the cellular uptake and metabolism of Ac₄ManNAz in Jurkat cells that are treated with Ac₄ManNAl (50 μM, 1 min) followed by Ac₄ManNAz (50 μM) treatment. The curve shows azide-dependent fluorescence at various time intervals. Data shown in (A) and (B) were obtained using biotin azide 7.