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. 2014 Feb 5;25(4):698–706. doi: 10.1021/bc400502d

Figure 5.

Figure 5

Monitoring the kinetics of fucoside biosynthesis in zebrafish embryos. Zebrafish embryos were microinjected with GDP-FucAl at the one-cell stage. At (A) 2-, (B) 4-, (C) 8-cell stages, and (D) 2 hpf of ZM2 treated embryos, embryos were fixed and reacted with Alexa Fluor 488 azide 8, and imaged using confocal microscopy. First row, side views of live embryos at time points equivalent to 2-, 4-, and 8-cell stages and 2 hpf of ZM2 treated embryos. Rows 2–4: animal pole views of fixed embryos labeled with 488. Nuclei were stained with Hoechst 33342.