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. 2014 Mar 26;25(4):707–717. doi: 10.1021/bc400507v

Figure 1.

Figure 1

NCL reaction scheme. The reaction mixture containing 1 mM CysPA (1), 1 mM peptide-Nbz (2), 5 mM 4-MPAA, and 5 mM DTT in PBS was reacted at pH 7.4 for 24 h at 37 °C to generate peptide-functionalized PA (3). The peptides used in the reaction are RGDS (2a,3a), biotin-RGDS (2b,3b), and RGES (2c,3c).