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. 2014 Jan 20;11(3):1022–1031. doi: 10.1021/mp400689r

Figure 3.

Figure 3

Cell viability of A549 (A, B) and HEK293 (C, D) cell lines treated with CSpp(CpG(−)) (A, C) and CSpp(CpG(+)) (B, D) was determined by MTS assay. Cells were treated with CSpp containing N/P ratios ranging from 1 to 100. CpG(−) represents soluble CpG-free plasmid DNA while CpG(+) represents soluble CpG-containing plasmid DNA. PEIss(CpG(−)) or PEIpp(CpG(+)) were PEI complexes with pDNA at N/P 10. Control = cells treated with PBS. The cells were seeded into a 96-well plate at a density of 1 × 104 cells per well one day prior the experiment and exposed to the treatments for 48 h. Each treatment contained 1 μg of pDNA per well. Data are expressed as mean ± SD (n = 3). Percent relative cell viability compared to cells treated with PBS. *** p < 0.001.