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. 2014 Jan 20;11(3):1022–1031. doi: 10.1021/mp400689r

Figure 5.

Figure 5

Transfection efficiency of A549 (A, B) and HEK293 (C, D) cells treated with CSpp(CpG(−)) (A, C) or CSpp(CpG(+)) (B, D). Varying N/P ratios are indicated. Control = cells treated with PBS. Each treatment contained 5 μg of pDNA/well. Data are expressed as mean ± SD (n = 3–7). * p < 0.05, ** p < 0.01, *** p < 0.001. PEIpp N/P 10 was used as a positive control for transfection (not shown in graphs). The luciferase expression of PEIpp-transfected A549 cells was equal to 2.32 × 1010 ± 7.45 × 109 RLU/mg (total protein) (for PEIpp(CpG(−)) and 2.54 × 1010 ± 1.62 × 1010 RLU/mg (for PEIpp(CpG(+)). The luciferase expression in HEK293 cells was 5.38 × 1010 ± 3.96 × 109 RLU/mg (for PEIpp(CpG(−)) and 4.23 × 1010 ± 2.92 × 1010 RLU/mg (for PEIpp(CpG(+)).