Table 1. Primer Sequences Used for Quantitative Reverse Transcription-Polymerase Chain Reaction Gene Expression Analysis.
genes | 5′-3′ | primers | product size (bp) |
---|---|---|---|
Smad8 | forward | CACGGCTTTGAAGTCGTGTAT | 198 |
reverse | TGAAGAAATGGGGTTATGTGGA | ||
Runx2 | forward | GTGATAAATTCAGAAGGGAGG | 118 |
reverse | CTTTTGCTAATGCTTCGTGT | ||
osteocalcin | forward | AGGGCAGCGAGGTAGTGAAGA | 181 |
reverse | TAGACCGGGCCGTAGAAGC | ||
integrin a2 | forward | CCGACAGGGGTTATCATAGGCA | 224 |
reverse | CATTCACCACACCAGCGAGC | ||
PPARγ | forward | TTCTCCTATTGACCCAGAAAGC | 307 |
reverse | CTCCACTTTGATTGCACTTTGG |