Table 1. Dissociation Constants of Various Zn-BDPA Compounds from PS-Richa and PC Vesiclesb in HEPES Bufferc at 25 °C.
| compound | Kd PS-rich vesicles, nMf | Kd PC vesicles, nMf |
|---|---|---|
| 12d | 133 ± 35 | 360 ± 60 |
| 13d | 39 ± 6 | 190 ± 31 |
| 11ke | 51 ± 6 | -- |
| 11oe | 37 ± 8 | -- |
a
PS-Rich vesicles were composed of POPS:POPC:14, 50:49:1.
b
PC vesicles were composed of POPC:14, 99:1.
c
HEPES buffer = 10 mM HEPES, 137 mM NaCl, 3.2 mM KCl, 1.0 mM Zn(NO3)2·6H2O, pH 7.4.
d
Kd determined using a FRET titration assay.
e
Kd determined using a FRET displacement assay.
f
Uncertainty is standard deviation from the mean for experiments performed in at least triplicate.