Table 1. Microfluidic (MF) and Conventional (CV) Radiolabeling of Peptide 13 with 64Cu(OAc)2.
radiolabeling method | temp (°C) | reaction scale/ [ligand] (μM) | reaction volume (μL) | total ligand mass (μg) | experimental L/M ratio | time (min) | HPLC labeling yield (%) | calculated SA (Ci/mmol) |
---|---|---|---|---|---|---|---|---|
MF | 37 | 4.5 | 52.6 | 0.5 | 2.1 | 10 | 97.4 ± 2 | 867.1 ± 88 |
MF | 37 | 4.6 | 52.6 | 0.5 | 7.5 | 10 | 97.8 ± 0.8 | 850.9 ± 84.9 |
CV | 90 | 9.1 | 192 | 3.7 | 62.3 | 30 | 99.0 | 535.5 |