Response curves for each peptide/affinity reagent.
The concentration
of heavy peptide was varied and measured relative to the light peptide
signal. One antibody fragment and full-length IgG was used for each
peptide, GDSLAYGLR (AbD 18303), NWAPGEPNNR (AbD 18279), and VDNEELLPK
(AbD 18307). For each peptide, the curve is plotted on a log 10 scale
and a linear scale. Curves obtained from using antibody fragments
(red), IgG (green), and rabbit or mouse monoclonal antibodies (purple)
are overlaid. Error bars are the standard deviation of three replicate
measurements.