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. 2014 Feb 25;13(4):2187–2196. doi: 10.1021/pr4009404

Figure 3.

Figure 3

Response curves for each peptide/affinity reagent. The concentration of heavy peptide was varied and measured relative to the light peptide signal. One antibody fragment and full-length IgG was used for each peptide, GDSLAYGLR (AbD 18303), NWAPGEPNNR (AbD 18279), and VDNEELLPK (AbD 18307). For each peptide, the curve is plotted on a log 10 scale and a linear scale. Curves obtained from using antibody fragments (red), IgG (green), and rabbit or mouse monoclonal antibodies (purple) are overlaid. Error bars are the standard deviation of three replicate measurements.