Table 2. Results of Antibody Generation for the Selections Using the Biotinylated Peptides in the Two RapMAT Panning Rounds^a.

gene symbol	protein description	peptide sequence	clones screened in ELISA	ELISA positive	binding to free peptide	clones sequenced	unique antibody sequences	affinity range (nM)
Spp1	osteopontin	GDSLAYGLR	1472	408 (28%)	81 (6%)	40	9	0.6–380
SELE	E-Selectin	NWAPGEPNNR	1472	325 (22%)	192 (13%)	42	20	0.4–243
ADA17	ADAM17 metalloprotease	VDNEELLPK	1472	577 (39%)	40 (3%)	33	17	33–91

^aClones with a signal at least five times above the background in ELISA on the respective biotinylated peptide captured by coated streptavidin were considered positive. The clones which in addition showed an at least five times lower signal on the biotinylated peptide in the presence of 10μM peptide with free amino- and C-termini were considered positive for binding to free peptide. For Spp1 and ADA17, also a few clones with less than five-fold signal reduction in the competition screening were sequenced. The affinity to the free peptide was determined for 5 (Spp1), 17 (SELE), and 5 antibodies (ADA17).