Table 4. Precision of Measurements at Each Concentration Point in the Response Curvea.
| %CV |
||||
|---|---|---|---|---|
| analyte | curve concentration (ng/mL) | Fab | IgG | Mab |
| osteopontin | 0.3 | 4.3 | 11.7 | |
| (GDSLAYGLR) | 1 | 6.5 | 6.2 | 8.9 |
| 4 | 8.2 | 13.0 | 8.5 | |
| 16 | 2.4 | 4.0 | 11.9 | |
| 65 | 1.6 | 2.7 | 6.5 | |
| 649 | 5.0 | 1.7 | 4.8 | |
| average | 4.7 | 6.5 | 8.1 | |
| E-selectin | 0.5 | 10.9 | 21.1 | 27.8 |
| (NWAPGEPNNR) | 2 | 3.9 | 11.1 | 4.1 |
| 8 | 11.8 | 9.6 | 8.2 | |
| 33 | 6.1 | 2.1 | 5.3 | |
| 133 | 0.5 | 2.2 | 4.9 | |
| 1333 | 3.2 | 2.8 | 3.3 | |
| average | 6.1 | 8.1 | 8.9 | |
| ADAM17 | 3 | 14.9 | 0.9 | |
| (VDNEELLPK) | 12 | 10.0 | 12.3 | |
| 47 | 1.9 | 7.7 | ||
| 186 | 5.8 | 2.6 | ||
| 1860 | 4.7 | 28.5 | 2.8 | |
| average | 7.5 | 28.5 | 5.3 | |
a
% CV is based on three replicate captures of peptide in 10 μL of plasma digest. Points above the limit of detection are reported. Protein concentration (ng/mL) is calculated assuming complete trypsin digestion.