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. 2014 Feb 3;13(3):1757–1765. doi: 10.1021/pr401280w

Figure 1.

Figure 1

Workflows tested in the discovery of novel human SEPs. (A) Schematic of the four different SEP discovery workflows used: MWCO+LC–MS; MWCO+ERLIC+LC–MS; PAGE+ERLIC+LC–MS; and PAGE+LC–MS. The K562 peptidome is separated by size using a 30 kDa MWCO filter (MWCO) or polyacrylamide gel electrophoresis (PAGE) and then analyzed directly by LC–MS (first and last lane) or fractionated by ERLIC prior to LC–MS analysis (middle lanes). (B) Number of total SEP and novel SEPs identified in K562 cells using each of the four different SEP discovery workflows.