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. Author manuscript; available in PMC: 2014 Apr 21.
Published in final edited form as: Arthritis Rheum. 2013 Oct;65(10):2594–2605. doi: 10.1002/art.38057

Figure 6.

Figure 6

Effects of SHP-1 inhibitor on the susceptibility of inflammatory macrophages and CD4 T cells to TRA-8–mediated and TRAIL–mediated cell apoptosis in rheumatoid arthritis patients with high PTPN6 expression. Mononuclear cells from synovial fluid (A) or peripheral blood (B) of patients with rheumatoid arthritis exhibiting high PTPN6 expression and resistance to TRA-8–induced apoptosis were pretreated with or without SSG for 3 days followed by TRA-8 or TRAIL treatment for 2 days. Cells were then analyzed by FACS. A. SSG increased the numbers of M1 macrophages with up-regulated death receptor 5 (DR5) expression, which could be depleted by TRA-8 but not TRAIL. B. SSG increased the numbers of Th1/17 and Th17 cells with up-regulated DR5 expression, which could be depleted by TRA-8 but not TRAIL. Values are the mean (± SEM) (n = 5 subjects per group). * = P < 0.05, TRA-8 treatment versus isotype control in the indicated cell populations. See Figure 1 for other definitions.