Figure 4. The pUR4 fibronectin polymerization inhibitor attenuates SMC proliferation.

SMC were incubated in in Medium 231 supplemented with smooth muscle growth supplement for up to 7 days. 30–60 min after seeding, pUR4 (•), III-11C (▴), or an equivalent volume of PBS (□) were added as described in Methods. Cells were incubated for the indicated time, then washed, fixed with 1% paraformaldehyde, stained with 0.5% crystal violet and the absorbance at 540 nm determined. The absorbance of PBS treated cells 24 h post seeding was set equal to 1. Data represents the mean of 3 separate experiments, and the error bars the s.e.m. *p<.05.