|
E. coli
strains
|
|
|
| DH5α |
F-, φ80d deoR lacZΔM15 endA1 recA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF) U169
|
Invitrogen |
|
C. jejuni
strains
|
|
|
| 81–176 |
Wild type isolated from a diarrheic patient |
[28]
|
| 81–176 pRRH |
Strain 81–176 with genome-integrated pRRH; HygR
|
This study |
| 81–176 pRRA |
Strain 81–176 with genome-integrated pRRA; AprR
|
This study |
| 81–176 pRRC |
Strain 81–176 with genome-integrated pRRC; CmR
|
This study |
| 81–176 pRRK |
Strain 81–176 with genome-integrated pRRK; KanR
|
This study |
| 81–176 ΔastA::hygR
|
Deletion of astA with aph(7″); HygR
|
This study |
| 81–176 ΔastA::aprR
|
Deletion of astA with aac(3)IV; AprR
|
This study |
| DRH461 |
Strain 81–176 with an unmarked deletion of astA
|
[19]
|
| DRH461 pRRH+astA
|
DRH461 with integrated pRRH and polycistronic promoterless astA; HygR
|
This study |
| DRH461 pRRA+astA
|
DRH461 with integrated pRRA and polycistronic promoterless astA; AprR
|
This study |
| DRH461 pRRH+astA (reverse) |
DRH461 with integrated pRRH and reverse orientation promoterless astA; HygR
|
This study |
| DRH461 pRRA+astA (reverse) |
DRH461 with integrated pRRA and reverse orientation promoterless astA; AprR
|
This study |
| DRH461 pRRH+(p)astA (reverse) |
DRH461 with integrated pRRH and reverse orientation endogenous promoter and astA; HygR
|
This study |
| DRH461 pRRA+(p)astA (reverse) |
DRH461 with integrated pRRA and reverse orientation endogenous promoter and astA; AprR
|
This study |
|
Plasmids
|
|
|
| pMV261.hyg |
Source of aph(7″); HygR
|
[29], [30]
|
| p261comp.apra |
Source of aac(3)IV; AprR
|
[30]
|
| pGEM-T |
Linearized cloning vector, blue-white screening; ApR
|
Novagen |
| pBAD24 |
Low-copy arabinose-inducible expression vector; ApR
|
[31]
|
| pAC1H |
pBAD24 ligated to aph(7″) amplified with 5631 and 5632; HygR, ApR
|
This study |
| pAC1A |
pGEM-T ligated to aac(3)IV amplified with 5633 and 5634; AprR, ApR
|
This study |
| pRRC |
C. jejuni vector for genome integration at rRNA loci; CmR
|
[18]
|
| pRRK |
C. jejuni vector for genome integration at rRNA loci; KanR
|
J. Ketley |
| pRRH |
C. jejuni vector for genome integration at rRNA loci; HygR
|
This study |
| pRRA |
C. jejuni vector for genome integration at rRNA loci; AprR
|
This study |
| pGEM-T+astA
|
pGEM-T ligated to astA amplified with 5707 and 5708; ApR
|
This study |
| pGEM-T+astA::hygR
|
pGEM-T with astA interrupted with aph(7″) from pAC1H; HygR, ApR
|
This study |
| pGEM-T+astA::aprR
|
pGEM-T with astA interrupted with aac(3)IV from pAC1A; AprR, ApR
|
This study |
| pRRH+astA
|
pRRH ligated to astA amplified with 0688 and 0689; HygR
|
This study |
| pRRA+astA
|
pRRA ligated to astA amplified with 0688 and 0689; AprR
|
This study |
| pRRH+astA (reverse) |
pRRH ligated to astA amplified with 0690 and 0691; HygR
|
This study |
| pRRA+astA (reverse) |
pRRA ligated to astA amplified with 0690 and 0691; AprR
|
This study |
| pRRH+(p)astA (reverse) |
pRRH ligated to astA amplified with 0692 and 0691; HygR
|
This study |
| pRRA+(p)astA (reverse) |
pRRA ligated to astA amplified with 0692 and 0691; AprR
|
This study |
