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. 2014 Apr 21;9(4):e95084. doi: 10.1371/journal.pone.0095084

Table 2. Oligonucleotides used in this study (with restriction sites underlined).

Primer Sequence 5′-3′ Target, sense anddescription Restrictionsites
5631 ACACCAATTGGGTACCCGGGTGACTAACTAGGAGGAATAAATGACACAAGAATCCCTGTTAC aph (7″), sense, startcodon changed toATG. MfeI, KpnI, SmaI
5632 GTGTGCATGCCTGCAGCATATGTCTAGAGGATCCCCGGGTCATTATTCCCTCCAGGTATCAGGCGCCGGGGGCGGTGTCC aph (7″), antisense. SmaI, BamHI, XbaI, NdeI, PstI, SphI
5633 ACACCAATTGGGTACCCGGGTGACTAACTAGGAGGAATAAATGCAATACGAATGGCGAAAAG aac (3)IV, sense,start codon changedto ATG. MfeI, KpnI, SmaI
5634 GTGTGCATGCCTGCAGCATATGTCTAGAGGATCCCCGGGTCATTATTCCCTCCAGGTATCAGCCAATCGACTGGCGAGCG aac (3)IV, antisense. SmaI, BamHI, XbaI, NdeI, PstI, SphI
5705 ACACGGTACCTCCTCCGTAAATTCCGATTTG pRRC cat,antisense. KpnI
5706 TGGATGAATTACAAGACTTGCTG pRRC cat, sense.
5707 TATAGGCGAACCAAAAAATCC Flanking astA,sense.
5708 AAATGTAAATTTGGAAAAGCTTCTC Flanking astA,antisense.
5709 ACACGGTACCGATCAATCCTTTAAAATTATTTAA 5′-internal astA,antisense. KpnI
5710 ACACTCTAGACAATAAGCCCAAAAATAAATTTGG 3″-internal astA,sense. XbaI
0688 ACACTCTAGATAAAGGATTGATCATGAGACTTAG Promoterless astA,sense. Forpolycistronic expression. XbaI
0689 ACACCAATTGATAAGCCCAAATTTATTTTTGGGC astA, antisense.For polycistronicexpression. MfeI
0690 ACACTCTAGATAAAGGATTGATCATGAGACTTAG Promoterless astA,sense. For reverseexpression. MfeI
0691 ACACCAATTGATAAGCCCAAATTTATTTTTGGGC astA, antisense.For reverseexpression. XbaI
0692 ACACTCTAGATATAGGCGAACCAAAAAATCC Upstream astA,sense. For reverseexpression. MfeI