Effects of chronic ethanol feeding and NAC treatment on steatohepatitis. Adult male Sprague-Dawley rats were fed for 130 days with chow (A, F, K), control TEN (B, G, L), control TEN supplemented with NAC (C, H, M), ethanol TEN (37% by caloric content; D, I, N), or ethanol TEN supplemented with NAC (E, J, O). Paraffin-embedded histological sections (5 µm thick) of liver were stained with H&E and photographed at 40× (A–E), 160× (F–J), and 400× (K–O) magnifications. Note reduced inflammation and shift from microvesicular steatosis to macrovesicular steatosis associated with NAC treatments, but persistence of hepatic chord architecture disarray in the control liquid + ethanol livers despite NAC treatment.