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Effects of chronic ethanol and NAC treatment on pro-inflammatory cytokine and CYP2E1 gene expression in liver. RNA extracted from liver was reverse transcribed and the cDNAs were used to measure to (A) IL-1β, (B) IL-2, (C) IL-6, (D) TNF-α, and (E) CYP2E1 by qPCR using gene-specific primers (Supplementary Table 1). Inter-group comparisons were made using one-way ANOVA with the Tukey multiple comparison tests. Significant P-values are indicated within the panels.
