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Effects of chronic ethanol and NAC treatment on insulin/IGF signaling network genes. RNA extracted from liver was reverse transcribed to measure (A) IGF-1, (B) insulin receptor, (C) IGF-1 receptor, (D) IGF-2 receptor, (E) IGF-2, (F) IRS-1, (G) IRS-2, and (H) IRS-4 mRNA expression by PCR. Inter-group comparisons were made using one-way ANOVA with the Tukey multiple comparison tests. Significant P-values are indicated within the panels.
